Yellow Fever Research Today is a free monthly online journal that collates and summarizes the latest research about Yellow Fever, including details on immunization, vaccines, symptoms, transmission. | ||||||
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Flavivirus NS3 and NS5 proteins interaction network: A high-throughput yeast two-hybrid screen.Le Breton M, Meyniel-Schicklin L, Deloire A, Coutard B, Canard B, de Lamballerie X, Andre P, Rabourdin-Combe C, Lotteau V, Davoust N
ABSTRACT: BACKGROUND: The genus Flavivirus encompasses more than 50 distinct species of arthropod-borne viruses, including several major human pathogens, such as West Nile virus, yellow fever virus, Japanese encephalitis virus and the four serotypes of dengue viruses (DENV type 1-4). Each year, flaviviruses cause more than 100 million infections worldwide, some of which lead to life-threatening conditions such as encephalitis or haemorrhagic fever. Among the viral proteins, NS3 and NS5 proteins constitute the major enzymatic components of the viral replication complex and are essential to the flavivirus life cycle. RESULTS: We report here the results of a high-throughput yeast two-hybrid screen to identify the interactions between human host proteins and the flavivirus NS3 and NS5 proteins. Using our screen results and literature curation, we performed a global analysis of the NS3 and NS5 cellular targets based on functional annotation with the Gene Ontology features. We finally created the first flavivirus NS3 and NS5 proteins interaction network and analysed the topological features of this network. Our proteome mapping screen identified 108 human proteins interacting with NS3 or NS5 proteins or both. The global analysis of the cellular targets revealed the enrichment of host proteins involved in RNA binding, transcription regulation, vesicular transport or innate immune response regulation. CONCLUSIONS: We proposed that the selective disruption of these newly identified host/virus interactions could represent a novel and attractive therapeutic strategy in treating flavivirus infections. Our virus-host interaction map provides a basis to unravel fundamental processes about flavivirus subversion of the host replication machinery and/or immune defence strategy. Published 21 October 2011 in BMC Microbiol, 11(1): 234. Articles on Yellow Fever published 20 October 2011: Malaria knowledge and long-lasting insecticidal net use in rural communities of central Côte d'Ivoire. Malar J, 10: 288. Articles on Yellow Fever published 19 October 2011: Several diseases are associated to the mosquito-human interaction. Mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikunguniya fever, West Nile virus, and yellow fever. These diseases produce significant morbidity and mortality in humans and livestock around the world. The adulticidal and repellent activities of crude hexane, ethyl acetate, benzene, chloroform, and methanol extracts of leaf of Eclipta alba and Andrographis ... [Abstract] [Full-text] Articles on Yellow Fever published 18 October 2011: Use of replication restricted recombinant vesicular stomatitis virus vectors for detection of antigen-specific T cells. J Immunol Methods. Detection of antigen-specific T cells at the single-cell level by ELISpot or flow cytometry techniques employing intracellular cytokine staining (ICS) is now an indispensable tool in many areas of immunology. When precisely mapped, optimal MHC-binding peptide epitopes are unknown, these assays use antigen in a variety of forms, including recombinant proteins, overlapping peptide sets representing one or more target protein sequences, microbial lysates, lysates of microbially-infected cells, or ... [Abstract] [Full-text] Articles on Yellow Fever published 17 October 2011: Yellow Fever Virus Vaccine-associated Deaths in Young Women. Emerg Infect Dis, 17(10): 1891-3. Yellow fever vaccine-associated viscerotropic disease is a rare sequela of live-attenuated virus vaccine. Elderly persons and persons who have had thymectomies have increased susceptibility. A review of published and other data suggested a higher than expected number of deaths from yellow fever vaccine-associated viscerotropic disease among women 19-34 years of age without known immunodeficiency. [Abstract] [Full-text] Articles on Yellow Fever published 14 October 2011: Malignancy and specific allergen immunotherapy: the results of a case series. Int Arch Allergy Immunol, 156(3): 313-9. Background: Specific immunotherapy with allergen is the only causative treatment for IgE-mediated allergies such as stinging insect allergy or hay fever and works by the induction of blocking antibodies and regulatory T lymphocytes. Objective: Does a hypothetical obstruction of tumor surveillance presupposing the induction of regulatory T cells really justify detaining immunotherapy to oncologic patients as suggested by recent guidelines? Methods: We report 6 patients (4 female, 2 male) ... [Abstract] [Full-text] Articles on Yellow Fever published 12 October 2011: Imaginal Discs - A New Source of Chromosomes for Genome Mapping of the Yellow Fever Mosquito Aedes aegypti. PLoS Negl Trop Dis, 5(10): e1335. Mosquito feeding affects larval behaviour and development in a moth. PLoS One, 6(10): e25658. Organisms are attacked by different natural enemies present in their habitat. While enemies such as parasitoids and predators will kill their hosts/preys when they successfully attack them, enemies such as micropredators will not entirely consume their prey. However, they can still have important consequences on the performance and ecology of the prey, such as reduced growth, increased emigration, disease transmission.In this paper, we investigated the impact of a terrestrial micropredator, the ... [Abstract] [Full-text] Articles on Yellow Fever published 11 October 2011: Yellow Fever vaccination and increased relapse rate in travelers with multiple sclerosis. Arch Neurol, 68(10): 1267-71. © 2005-2011 Yellow Fever Research Today. All Rights Reserved. |
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